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Ligand‐specific Dose–response of Heterologously Expressed Olfactory Receptors by Grégoire Levasseur; Marie‐Annick Persuy; Denise Grebert; Jean‐Jacques Remy; Roland Salesse; Edith Pajot‐Augy is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Ligand‐specific Dose–response of Heterologously Expressed Olfactory Receptors about?

Primary olfactory neuronal cultures exposed to odorant stimulation have previously exhibited concentration‐related effects in terms of intracellular cAMP levels and adenylate cyclase activity [Ronnett, G.V., Parfitt, D.J., Hester, L.D. & Snyder, S.H. (1991) __PNAS__**88**, 2366–2369]. Maximal stimulation occurred for intermediate concentrations, whereas AC activity declined for both low and high odorant concentrations. We suspected that this behavior might be ascribed to the intrinsic response of the first molecular species concerned by odorant detection, i.e. the olfactory receptor itself. In order to check this hypothesis, we developed an heterologous expression system in mammalian cells to characterize the functional response of receptors to odorants. Two mammalian olfactory receptors were used to initiate the study, the rat I7 olfactory receptor and the human OR17‐40 olfactory receptor. The cellular response of transfected cells to an odorant stimulation was tested by a spectrofluorimetric intracellular calcium assay, and proved in all cases to be dose‐dependent for the known ligands of these receptors, with an optimal response for intermediate concentrations. Further experimen

Who reads Ligand‐specific Dose–response of Heterologously Expressed Olfactory Receptors?

Author

Grégoire Levasseur; Marie‐Annick Persuy; Denise Grebert; Jean‐Jacques Remy; Roland Salesse; Edith Pajot‐Augy

Publisher

John Wiley and Sons; Springer; Wiley (Blackwell Publishing); Springer-Verlag; Blackwell Publishing Inc.; Springer Verlag; Wiley; Springer Science and Business Media LLC (ISSN 1432-1327)

Published

2003

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)