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Tug of War between Condensate Phases in a Minimal Macromolecular System by Archishman Ghosh; Xiaojia Zhang; Huan-Xiang Zhou is a Chemistry article available to read on EtoBox.

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Membraneless organelles, comprising dozens to hundreds of macromolecular components, form heterogeneous phases in space and evolve over time in material properties. Here, using four macromolecules, we demonstrate a range of phase behaviors associated with membraneless organelles and uncover the underlying physicochemical rules. The macromolecules are SH3 (S) and PRM (P), two pentameric, oppositely charged protein constructs; heparin (H), an anionic polymer; and lysozyme (L), a cationic single-domain protein. The S:P, S:L, and P:H binaries form droplets, but the H:L binary forms network-like precipitates, therefore setting up a tug of war between different condensate phases within the S:P:H:L quaternary. The H:L exception can partly be attributed to the compactness of L, as supported by ThT binding data. Increasing amounts of P alone or both S and P, but not S alone, can dissolve H:L precipitates into droplets. These differential effects can be explained by the order of the strengths of pairwise attraction: H:L > P:H > S:P > S:L, deduced from the shapes of ternary phase boundaries. When S and P are at subdissolution concentrations, S:P:H:L precipitates change over time to become dro

Who reads Tug of War between Condensate Phases in a Minimal Macromolecular System?

Author

Archishman Ghosh; Xiaojia Zhang; Huan-Xiang Zhou

Publisher

American Chemical Society; American Chemical Society (ACS); Research Square (ISSN 0002-7863)

Published

2020

Language

EN

Field

Chemistry (Physical Sciences)