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Evidence for a Positive Role of Transforming Growth Factor-β in Human Breast Cancer Cell Tumorigenesis by Carlos L. Arteaga; Teresa C. Dugger; Angela R. Winnier; James T. Forbes is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Evidence for a Positive Role of Transforming Growth Factor-β in Human Breast Cancer Cell Tumorigenesis about?

To determine the biological role of transforming growth factor-@ (TGF-@) in mammary carcinomas in vim, estrogen-dependent MCF-7 cells were transfected with a mouse TGF-@I cDNA. Growth characteristics in culture were not altered in the transfected cells. However, the MCF-7/TGF-@1 cells formed tumors in ovariectomized athymic mice in the absence of estrogen supplementation. Daily injections of human recombinant TGF-@I supported tumor formation by wild-type MCF-7 cells in castrated nude mice in the absence of exogenous estradiol. In another approach to the same question, the effect of anti-TGF-@ antibodies on tumor formation by estrogen-independent MDA-231 cells was examined. The 2G7 IgG2b (2G7) antibody, which neutralizes TGF-@I, 42, and 43, blocked the formation of MDA-231 tumors at the injection site and lung metastases in nude mice. Inoculation of MDA-231 cells inhibited, while injection of 2G7 increased mouse spleen natural killer (NK) activity. 2G7 did not inhibit MDA-231 tumors and metastases in NK-deficient animals. Finally, medium conditioned by MDA-231 cells inhibited lymphocyte-mediated NK activity; this inhibition was abrogated by 2G7, but not by a control IgG2. These data

Who reads Evidence for a Positive Role of Transforming Growth Factor-β in Human Breast Cancer Cell Tumorigenesis?

Author

Carlos L. Arteaga; Teresa C. Dugger; Angela R. Winnier; James T. Forbes

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0730-2312)

Published

1993

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)