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Generation of a single-cell B cell atlas of antibody repertoires and transcriptomes to identify signatures associated with antigen specificity by Andreas Agrafiotis; Daniel Neumeier; Kai-Lin Hong; Tasnia Chowdhury; Roy Ehling; Raphael Kuhn; Ioana Sandu; Victor Kreiner; Tudor-Stefan Cotet; Danielle Shlesinger; Daria Laslo; Stine Anzböck; Dale Starkie; Daniel J. Lightwood; Annette Oxenius; Sai T. Reddy; Alexander Yermanos is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Generation of a single-cell B cell atlas of antibody repertoires and transcriptomes to identify signatures associated with antigen specificity about?

Although new genomics-based pipelines have potential to augment antibody discovery, these methods remain in their infancy due to an incomplete understanding of the selection process that governs B cell clonal selection, expansion, and antigen specificity. Furthermore, it remains unknown how factors such as aging and reduction of tolerance influence B cell selection. Here we perform single-cell sequencing of antibody repertoires and transcriptomes of murine B cells following immunizations with a model therapeutic antigen target. We determine the relationship between antibody repertoires, gene expression signatures, and antigen specificity across 100,000 B cells. Recombinant expression and characterization of 227 monoclonal antibodies revealed the existence of clonally expanded and class-switched antigen-specific B cells that were more frequent in young mice. Although integrating multiple repertoire features such as germline gene usage and transcriptional signatures failed to distinguish antigen-specific from nonspecific B cells, other features such as immunoglobulin G (IgG) subtype and sequence composition correlated with antigen specificity.

Who reads Generation of a single-cell B cell atlas of antibody repertoires and transcriptomes to identify signatures associated with antigen specificity?

Author

Andreas Agrafiotis; Daniel Neumeier; Kai-Lin Hong; Tasnia Chowdhury; Roy Ehling; Raphael Kuhn; Ioana Sandu; Victor Kreiner; Tudor-Stefan Cotet; Danielle Shlesinger; Daria Laslo; Stine Anzböck; Dale Starkie; Daniel J. Lightwood; Annette Oxenius; Sai T. Reddy; Alexander Yermanos

Publisher

Elsevier BV

Published

2023

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)