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The document describes an improved method for detecting amylase activity using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) with copolymerized starch, which allows for simultaneous identification of amylase bands and molecular mass determination. This technique prevents hydrolysis of the substrate during electrophoresis by maintaining low temperatures and is applicable to a variety of biological samples, although it is not suitable for SDS-sensitive enzymes or high molecular mass amy
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- srinidhidas.bvks
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- EN