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Autoantibodies identify primary Sjögren’s syndrome in patients lacking serum IgG specific for Ro/SS-A and La/SS-B by Sherri Longobardi; Charmaine Lopez-Davis; Bhuwan Khatri; Constantin Georgescu; Cherilyn Pritchett-Frazee; Christina Lawrence; Astrid Rasmussen; Lida Radfar; Robert Hal Scofield; Alan N Baer; Susan A Robinson; Erika Darrah; Robert C Axtell; Gabriel Pardo; Jonathan D Wren; Kristi A Koelsch; Joel M Guthridge; Judith A James; Christopher J Lessard; Amy Darise Farris is a Medicine article available to read on EtoBox.

What is Autoantibodies identify primary Sjögren’s syndrome in patients lacking serum IgG specific for Ro/SS-A and La/SS-B about?

Objective Identify autoantibodies in anti-Ro/SS-A negative primary Sjögren’s syndrome (SS). Methods This is a proof-of-concept, case-control study of SS, healthy (HC) and other disease (OD) controls. A discovery dataset of plasma samples (n=30 SS, n=15 HC) was tested on human proteome arrays containing 19 500 proteins. A validation dataset of plasma and stimulated parotid saliva from additional SS cases (n=46 anti-Ro+, n=50 anti-Ro–), HC (n=42) and OD (n=54) was tested on custom arrays containing 74 proteins. For each protein, the mean+3 SD of the HC value defined the positivity threshold. Differences from HC were determined by Fisher’s exact test and random forest machine learning using 2/3 of the validation dataset for training and 1/3 for testing. Applicability of the results was explored in an independent rheumatology practice cohort (n=38 Ro+, n=36 Ro–, n=10 HC). Relationships among antigens were explored using Search Tool for the Retrieval of Interacting Genes/Proteins (STRING) interactome analysis. Results Ro+SS parotid saliva contained autoantibodies binding to Ro60, Ro52, La/SS-B and muscarinic receptor 5. SS plasma contained 12 novel autoantibody specificities, 11 of whic

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Author

Sherri Longobardi; Charmaine Lopez-Davis; Bhuwan Khatri; Constantin Georgescu; Cherilyn Pritchett-Frazee; Christina Lawrence; Astrid Rasmussen; Lida Radfar; Robert Hal Scofield; Alan N Baer; Susan A Robinson; Erika Darrah; Robert C Axtell; Gabriel Pardo; Jonathan D Wren; Kristi A Koelsch; Joel M Guthridge; Judith A James; Christopher J Lessard; Amy Darise Farris

Publisher

BMJ

Published

2023

Language

EN

Field

Medicine (Health Sciences)