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Abundance and subcellular localisation of cyclin D3 in human tumours by Jirina Bartkova; Marie Zemanova; Jiri Bartek is a Medicine article available to read on EtoBox.

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The D-type cyclins are positive regulators of the GI phase of the mammalian cell cycle. Cyclins D I or D2 are over-expressed in several types of cancer, transform rodent cells in culture and therefore harbor hallmarks of cellular proto-oncogenes. In contrast, no data on expression of cyclin 0 3 in tissues and tumours are presently available. We have raised monoclonal antibodies (MAbs) specific for cyclin D3 and examined abundance and subcellular localisation of this GI cyclin in a series of human cultured cell types and in I80 primary tumours of diverse histogenesis. Cyclin D3 localised predominantly in nuclei of normal and tumour cells both in culture and in situ, and a pronounced cell-to-cell variation of its abundance was reminiscent of cyclins D I and D2. Immunohistochemical analysis of tumour and corresponding normal tissues showed strong aberrant accumulation of cyclin D3 in a subset (about 10%) of breast carcinomas, whereas only weak-to-moderate expression was found in colorectal, head and neck and uterine carcinomas, melanomas and soft tissue sarcomas. The specificity of the immunohistochemical data was confirmed by immunoblotting analysis of tissue and tumour lysates. Our

Who reads Abundance and subcellular localisation of cyclin D3 in human tumours?

Author

Jirina Bartkova; Marie Zemanova; Jiri Bartek

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square; Test accounts (ISSN 0020-7136)

Published

1996

Language

EN

Field

Medicine (Health Sciences)