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What is Updated Gradient about?

The document outlines two different gradient protocols for a total duration of 30 minutes each, detailing the percentage of Buffer (A) and Methanol (B) at specified time intervals. Key remarks include holding conditions for niacinamide, the separation zones for vitamins B6, B2, and B1, and the re-equilibration phases. The protocols aim to optimize the elution and separation of specific vitamins during the process.

Author

Anand H S

Language

EN