Can I read Putrescine-Modified Nerve Growth Factor: Bioactivity, Plasma Pharmacokinetics, Blood-Brain/Nerve Barrier Permeability, and Nervous System Biodistribution on EtoBox?

Putrescine-Modified Nerve Growth Factor: Bioactivity, Plasma Pharmacokinetics, Blood-Brain/Nerve Barrier Permeability, and Nervous System Biodistribution by Joseph F. Poduslo; Geoffry L. Curran; Jagjit S. Gill is a Neuroscience article available to read on EtoBox.

What is Putrescine-Modified Nerve Growth Factor: Bioactivity, Plasma Pharmacokinetics, Blood-Brain/Nerve Barrier Permeability, and Nervous System Biodistribution about?

Abstract: Previous investigations from our laboratory have demonstrated that the covalent modification of a variety of proteins, including antioxidant enzymes, with the naturally occurring polyamines—putrescine (PUT), spermidine, and spermine—dramatically increases their permeability coefficient‐surface area product (__PS__) at the blood‐brain and blood‐nerve barriers after parenteral administration. In the present study, we have covalently modified nerve growth factor (NGF) with PUT by targeting carboxylic groups for their graded modification by controlling the ionization of these groups with pH. Sodium dodecyl sulfate‐polyacrylamide gel electrophoresis, western, and isoelectric focusing analyses demonstrated conversion of NGF to its polyamine‐modified derivatives at different pH values. Although the immunoreactivity of PUT‐NGF determined by ELISA and western analysis decreased with decreasing pH, the biological activity of PUT‐NGF was not affected at any pH as determined by survival and neurite extension of dorsal root ganglia and PC12 cultures. Plasma pharmacokinetics after a single intravenous bolus administration revealed intact PUT‐NGF through 10 min and 73–82% intact protein

Who reads Putrescine-Modified Nerve Growth Factor: Bioactivity, Plasma Pharmacokinetics, Blood-Brain/Nerve Barrier Permeability, and Nervous System Biodistribution?

Author

Joseph F. Poduslo; Geoffry L. Curran; Jagjit S. Gill

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0022-3042)

Published

1998

Language

EN

Field

Neuroscience (Life Sciences)