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Structural analysis of lipopolysaccharide oligosaccharide epitopes expressed by non-typeable Haemophilus influenzae strain 176 by Elke K.H Schweda; Jianjun Li; E.Richard Moxon; James C Richards is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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The structure of the lipopolysaccharide (LPS) from non-typeable Haemophilus influenzae strain 176 has been investigated. Electrospray ionization-mass spectrometry (ESIMS) on O-deacylated LPS (LPS-OH) and core oligosaccharide (OS) samples obtained after mild-acid hydrolysis of LPS provided information on the composition and relative abundance of the glycoforms. ESIMS tandem-mass spectrometry on LPS-OH confirmed the presence of minor sialylated and disialylated glycoforms. Oligosaccharide samples were studied in detail using high-field NMR techniques. It was found that the LPS contains the common inner-core element of H. influenzae, L-a-D-Hepp -(1 2)-[PEtn 6]-L-a-D-Hepp-(1 3)-[b-D-Glcp-(1 4)]-L-a-D-Hepp-(1 5)-[PPEtn 4]-a-Kdop-(2 6)-Lipid A having glycosyl substitution at the O-3 position of the terminal heptose as recently observed for non-typeable H. influenzae strain 486 [

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Author

Elke K.H Schweda; Jianjun Li; E.Richard Moxon; James C Richards

Publisher

Elsevier Science; Elsevier ; Elsevier Ltd.; Elsevier BV (ISSN 0008-6215)

Published

2002

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)