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Alterations of the intestinal mucus layer correlate with dysbiosis and immune dysregulation in human Type 1 Diabetes by Marta Lo Conte; Ilaria Cosorich; Roberto Ferrarese; Martina Antonini Cencicchio; Angelica Nobili; Vittoria Palmieri; Luca Massimino; Luigi Antonio Lamparelli; Wenjie Liang; Michela Riba; Elisabetta Devecchi; Andrea Mario Bolla; Erika Pedone; Marina Scavini; Emanuele Bosi; Alessio Fasano; Federica Ungaro; Julien Diana; Nicasio Mancini; Marika Falcone is a Medicine article available to read on EtoBox.

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## Summary ## Background In preclinical models of Type 1 Diabetes (T1D) the integrity of the gut barrier (GB) is instrumental to avoid dysregulated crosstalk between the commensal microbiota and immune cells and to prevent autoimmunity. The GB is composed of the intestinal epithelial barrier (IEB) and of the mucus layer containing mucins and antimicrobial peptides (AMPs) that are crucial to maintain immune tolerance. In preclinical models of T1D the alterations of the GB primarily affect the mucus layer. In human T1D increased gut permeability and IEB damage have been demonstrated but the integrity of the mucus layer was never assessed. ## Methods We evaluated GB integrity by measuring serological markers of IEB damage (serological levels of zonulin) and bacterial translocation such as lipopolysaccharide binding protein (LBP) and myeloid differentiation protein 2 (MD2), and mRNA expression of tight junction proteins, mucins and AMPs in intestinal tissue of T1D patients and healthy controls (HC). Simultaneously, we performed immunological profiling on intestinal tissue and 16S rRNA analysis on the mucus-associated gut microbiota (MAGM). ## Findings Our data show a GB damage with muc

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Author

Marta Lo Conte; Ilaria Cosorich; Roberto Ferrarese; Martina Antonini Cencicchio; Angelica Nobili; Vittoria Palmieri; Luca Massimino; Luigi Antonio Lamparelli; Wenjie Liang; Michela Riba; Elisabetta Devecchi; Andrea Mario Bolla; Erika Pedone; Marina Scavini; Emanuele Bosi; Alessio Fasano; Federica Ungaro; Julien Diana; Nicasio Mancini; Marika Falcone

Publisher

Elsevier

Published

2023

Language

EN

Field

Medicine (Life Sciences)