About this document

IHC Techniques and Sample Preparation Guide by Sharmishtha Shekhar is a document available to read on EtoBox.

Immunohistochemistry (IHC) is a technique that combines anatomical, immunological, and biochemical methods to identify specific cellular components in tissues through the interaction of target antigens with labeled antibodies. The key steps in IHC involve sample preparation, which includes tissue collection, fixation, embedding, sectioning and mounting, followed by epitope retrieval and blocking to reduce nonspecific binding. Antigen detection then occurs through direct or indirect antibody labeling and vis

Author

Sharmishtha Shekhar

Language

EN