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Inhibition of osteoclast differentiation and bone resorption by cathepsin K antisense oligonucleotides by Takashi Ishikawa; Masako Kamiyama; Nobuyuki Tani-Ishii; Hiroaki Suzuki; Yasushi Ichikawa; Yohei Hamaguchi; Nobuyoshi Momiyama; Hiroshi Shimada is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Inhibition of osteoclast differentiation and bone resorption by cathepsin K antisense oligonucleotides about?
## Abstract We confirmed the expression of cathepsin K, the most abundant and specific cysteine protease found in osteoclasts, at the mRNA level in most of our cases of breast cancer, and even at the protein level in bone metastatic lesions. Therefore, we investigated the functions of cathepsin K in osteoclasts with special attention to bone metastasis from breast cancer. Mouse osteoclast‐like cells (OCLs) were established by coculture of mouse bone marrow cells and osteoblastic cells. Rodent cathepsin K antisense (AS) or random control (CL) oligonucleotides were added on day 0, 3, or 6 of culture. Tartrate‐resistant acid phosphatase staining confirmed the formation of OCLs after 9 d of incubation. AS treatment significantly reduced both the number of TRAP‐positive cells and the percentage of multinuclear cells. For the pit‐forming assay, after 9 d of incubation, mature OCLs were collected and incubated on ivory slices with AS or CL for 48 h. The antisense oligonucleotides also inhibited the bone‐resorbing activity of OCLs. CL treatment did not affect either the number of TRAP‐positive cells or pit formation. Cathepsin K may play important roles in bone resorption as well as in dif
Who reads Inhibition of osteoclast differentiation and bone resorption by cathepsin K antisense oligonucleotides?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Takashi Ishikawa; Masako Kamiyama; Nobuyuki Tani-Ishii; Hiroaki Suzuki; Yasushi Ichikawa; Yohei Hamaguchi; Nobuyoshi Momiyama; Hiroshi Shimada
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0899-1987)
- Published
- 2001
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)