Can I read A General Method to Isolate Genes Tagged by a High Copy Number Transposable Element on EtoBox?

A General Method to Isolate Genes Tagged by a High Copy Number Transposable Element by Erik Souer; Francesca Quattrocchio; Nick De Vetten; Joseph Mol; Ronald Koes is a Agricultural and Biological Sciences article available to read on EtoBox.

What is A General Method to Isolate Genes Tagged by a High Copy Number Transposable Element about?

The Petunia hybrida line W138 contains more than 200 copies of the transposable element dTph1. In W138 progeny these elements give rise to new unstable mutations at high frequency. With the aim of isolating these mutated genes a method was developed to isolate dTph1 flanking sequences unique for mutant plants. This method is based on differential screening of cloned inverse polymerase chain reaction (IPCR) products originating from the mutated plant. It directly yields a probe for the mutated gene which can be used to screen pre-existing cDNA and genomic libraries. This method may be generally applicable to isolate genes tagged by other high copy number transposable elements, like Mutator (Mu) or Dissociation (Ds) in Zea mays.

Who reads A General Method to Isolate Genes Tagged by a High Copy Number Transposable Element?

Author

Erik Souer; Francesca Quattrocchio; Nick De Vetten; Joseph Mol; Ronald Koes

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0960-7412)

Published

1995

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)