Can I read Overproduction of Sweet Potato Peroxidases in Transgenic Tobacco Plants on EtoBox?

Overproduction of Sweet Potato Peroxidases in Transgenic Tobacco Plants by Gyung-Hye Huh; Byung-Wook Yun; Haeng-Soon Lee; Jin-Ki Jo; Sang-Soo Kwak is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Overproduction of Sweet Potato Peroxidases in Transgenic Tobacco Plants about?

Two peroxidase (POD) cDNAs, anionic (swpal) and neutral (swpnl) POD derived from suspension cultures of sweet potato (Ipomoea batatas), were used to express high levels in transgenic plants. Two sweet potato PODs were overproduced in two cultivars of transformed tobacco (Nicotiana tabacum) plants, cv. Bel W3 and cv. Samsun by introducing a chimeric gene composed of the cauliflower mosaic virus 35S promoter and POD cDNA. Young fully expanded leaves of transgenic plants of both cultivars showed higher POD activity than those of nontransgenic plants. Bel W3 transgenic plants carrying anionic POD had total POD activity ca five times higher than control plants. However, no significant differences were observed in superoxide dismutase activity between transgenic and nontransgenic plants. Transgenic Bel W3 and Samsun tobacco plants with either anionic or neutral POD isoenzyme did not show distinctive phenotypes compared with untransformed plants.

Who reads Overproduction of Sweet Potato Peroxidases in Transgenic Tobacco Plants?

Author

Gyung-Hye Huh; Byung-Wook Yun; Haeng-Soon Lee; Jin-Ki Jo; Sang-Soo Kwak

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0031-9422)

Published

1998

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)