Can I read Interphase cytogenetic analysis of myxoid soft tissue tumors by fluorescence in situ hybridization and DNA flow cytometry using paraffin-embedded tissue on EtoBox?
Interphase cytogenetic analysis of myxoid soft tissue tumors by fluorescence in situ hybridization and DNA flow cytometry using paraffin-embedded tissue by Takatoshi Aoki; Masanori Hisaoka; Hiromi Kouho; Hiroshi Hashimoto; Hajime Nakata is a Medicine article available to read on EtoBox.
What is Interphase cytogenetic analysis of myxoid soft tissue tumors by fluorescence in situ hybridization and DNA flow cytometry using paraffin-embedded tissue about?
## Background: Myxoid liposarcoma is one of the major myxoid malignancies of soft tissue and its histologic differentiation from other myxoid tumors is sometimes difficult. recent cytogenetic analysis revealed that a characteristic reciprocal chromosomal translocation of t(12;16)(q13;p11) occurs in myxoid liposarcomas. in this study, retrospective cytogenetic analysis by means of fluorescence in situ hybridization (fish) and dna flow cytometry were performed on free nuclei isolated from paraffin embedded myxoid liposarcoma cells, and they were compared with myxoid malignant fibrous histiocytomas (mfhs), low grade fibromyxoid sarcomas, and intramuscular myxomas. ## Methods: Nine myxoid liposarcomas, five myxoid mfhs, two low grade fibromyxoid sarcomas, and four intramuscular myxomas were examined. chromosomal aberrations in chromosomes 12 and 16 were investigated by fish, using both centromeric dna probes and whole chromosome painting probes. cellular dna contents were determined by flow cytometry. ## Results: All nine myxoid liposarcomas were shown to be diploid by dna flow cytometry and exhibited the translocation t(12; 16), as shown by fish. four of the five myxoid mfhs exhibited
Who reads Interphase cytogenetic analysis of myxoid soft tissue tumors by fluorescence in situ hybridization and DNA flow cytometry using paraffin-embedded tissue?
It is typically read by researchers, students, and practitioners in Medicine.
- Author
- Takatoshi Aoki; Masanori Hisaoka; Hiromi Kouho; Hiroshi Hashimoto; Hajime Nakata
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0008-543X)
- Published
- 1997
- Language
- EN
- Field
- Medicine (Health Sciences)