Can I read Citrate Ratio in Collection of Peripheral Blood Progenitors on EtoBox?

Citrate Ratio in Collection of Peripheral Blood Progenitors by Jeb B. Gorlin; Scott D. Rowley is a Medicine article available to read on EtoBox.

What is Citrate Ratio in Collection of Peripheral Blood Progenitors about?

In a recent issue of TRANSFUSION, Burger et al.' documented the potential for clot formation in peripheral blood progenitor cell collections. We suggest an alternative to a strict adherence to lower citrate ratios for those institutions that collect cells by using either the Spectra (COBE BCT, Lakewood, CO) or, potentially, an apheresis device from Haemonetics (Braintree, MA). The addition of heparin or ACD-A to the collection bag before initiation of the collection prevents clot formation.2 We have preferred the addition of ACD-A at 10 to 20 percent of the anticipated final volume, as heparin does not prevent platelet clumping that may affect the final mononuclear cell yield, especially if the collected component is subjected to additional processing steps3This technique with the Spectra has successfully prevented clot formation in over 2500 collections at the Fred Hutchinson Cancer Center and over 300 pediatric collections at Boston Children's Hospital. That the addition of ACD-A before collection does not cause any loss of viability has been validated by both functional assays (colony-forming units-granulocyte-macrophage and fluorescenceactivated cell-sorting viability assay^).^

Who reads Citrate Ratio in Collection of Peripheral Blood Progenitors?

Author

Jeb B. Gorlin; Scott D. Rowley

Publisher

Elsevier Science; John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Elsevier; Elsevier BV; Wiley (ISSN 0372-1248)

Published

1997

Language

EN

Field

Medicine (Health Sciences)