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Varying very low-density lipoprotein secretion of rat hepatocytes by altering cellular levels of calcium and the activity of protein kinase C by Björnsson; Bourgeois; Gibbons is a Medicine article available to read on EtoBox.
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## Background Calcium antagonists lower plasma levels of lipoproteins and suppress hepatic very low‐density lipoprotein (VLDL) secretion. Similar effects have been observed with the calcium ionophore A23187. We studied further the effect of calcium on VLDL metabolism. ## Methods Hepatocytes from male Wistar rats were isolated and cultured in the presence or absence of calcium‐mobilizing hormones, or compounds that either stimulate or inhibit the activity of protein kinase C. Secreted VLDL (__d__ 2.4 mmol L^−1^) suppressed both lipoprotein secretion and cellular levels of lipids, suggesting a reduced overall rate of lipid synthesis. At an extracellular calcium concentration of 0.8 mmol L^−1^, angiotensin II, vasopressin, endothelin‐1 (10^−7^ mol L^−1^) or phenylephrine (10^−4^ mol L^−1^) suppressed VLDL secretion (maximum to 37% of control), and elevated medium calcium attenuated this effect. The protein kinase C inhibitor chelerythrine (5 × 10^−5^ mol L^−1^) and the protein kinase C activator phorbol 12‐myristate 13‐acetate (PMA) (10^−6^ mol L^−1^), suppressed VLDL secretion to 18% and 60% of control, respectively, whereas the protein kinase C‐inactive 4α‐PMA was without an effec
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- Author
- Björnsson; Bourgeois; Gibbons
- Publisher
- John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0014-2972)
- Published
- 1998
- Language
- EN
- Field
- Medicine (Health Sciences)