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A procedure for isolation of spontaneous mutants with temperature sensitive synthesis of RNA and/or protein by Leif A. Isaksson; Sven-Erik Sköld; Jan Skjöldebrand; Renkichi Takata is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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A procedure for the isolation of spontaneous temperature sensitive mutants of Escherichia coli has been developed. They are selected as survivors at high temperature against the combined killing effects exerted by a temperature inducible lambda prophage and either streptomycin plus ampillicin or ampicillin plus cycloserine. The mutants so obtained are blocked in vivo in the synthesis of RNA or protein or both at restrictive temperature.

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Author

Leif A. Isaksson; Sven-Erik Sköld; Jan Skjöldebrand; Renkichi Takata

Publisher

Springer; Springer-Verlag; Springer Verlag; Springer Science and Business Media LLC (ISSN 0026-8925)

Published

1977

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)