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Proportional equilibration of K, Na ions, and sucrose molecules in pig lenses incubated in the presence of the non‐ionic detergent triton X‐100 by A. Miseta; M. Kellermayer; A. Ludany; I. L. Cameron; C. F. Hazlewood is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Proportional equilibration of K, Na ions, and sucrose molecules in pig lenses incubated in the presence of the non‐ionic detergent triton X‐100 about?

The release of sodium and potassium and the uptake of sucrose molecules was studied in pig lenses incubated in isosmotic sucrose solution in either the presence or absence of 1 % Triton X-100 (a non-ionic detergent). This Triton X-100 treatment has been shown to cause severe disruptions of cell membrane integrity. If sodium and potassium were free in the lens fibers as in a dilute aqueous solution, they would be expected to diffuse three to four times faster than sucrose. However, measurements of sodium and potassium release and sucrose uptake in the Triton X-100 treated lenses show a 1:l equilibration. When pig lenses were incubated in the same solution without detergent, the sucrose uptake was significantly less than the potassium and sodium release. It is postulated that a slow, detergent mediated collapse of protein-water-ion interactions within the lens is the rate-limiting step of the observed equilibration of monovalent cations and sucrose molecules.

Who reads Proportional equilibration of K, Na ions, and sucrose molecules in pig lenses incubated in the presence of the non‐ionic detergent triton X‐100?

Author

A. Miseta; M. Kellermayer; A. Ludany; I. L. Cameron; C. F. Hazlewood

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0021-9541)

Published

1991

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)