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Regulation of hexose transporters in chicken embryo fibroblasts: Stimulation by the phorbol ester TPA leads to increased numbers of functioning transporters by Kazuo Yamada; Loyal G. Tillotson; Kurt J. Isselbacher is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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As has been observed with many types of cultured cells, chicken embryo fibroblasts (CEF) when exposed to the tumor promoter 12-0-tetradecanoylphorbol-13-acetate (TPA) develop a 3-to 4-fold increase in hexose transport activity in 4 h. This increase in transport activity occurred despite a modest decline of 20% in [3H]leucine incorporation into acid insoluble fractions. Cycloheximide largely, but not completely, blocked the increase in transport activity during TPA exposure. The effects of TPA were somewhat similar to those of glucose starvation induced enhancement of hexose transport activity. Furthermore, with TPA there was no additive effect to that produced by glucose starvation. Plasma membrane enriched fractions were prepared from CEF treated with or without TPA. Membranes prepared from TPA exposed cells had a two-fold enhancement of stereospecific D-glucose transport activity as well as D-glucose inhibitable [3H]cytochalasin B binding as compared to the membranes from control CEF. There was n o effect on transport when membranes were exposed to TPA in vitro. These results provide strong evidence that TPA exposure leads to an increase in the number of functioning transporters,

Who reads Regulation of hexose transporters in chicken embryo fibroblasts: Stimulation by the phorbol ester TPA leads to increased numbers of functioning transporters?

Author

Kazuo Yamada; Loyal G. Tillotson; Kurt J. Isselbacher

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0021-9541)

Published

1986

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)