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Characteristics of a label-free piezoelectric immunosensor detecting Pseudomonas aeruginosa by Namsoo Kim; In-Seon Park; Dong-Kyung Kim is a Engineering article available to read on EtoBox.

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A label-free immunosensor system detecting a psychrophylic bacterium, Pseudomonas aeruginosa was developed as follows. Four types of anti-P. aeruginosa antibody were individually chemisorbed onto one-side gold electrodes of piezoelectric quartz crystals according to a thiolated antibody coupling procedure initiated with a thiol-cleavable heterobifunctional cross-linker, sulfosuccinimidyl-6-[3-(2-pyridyldithio)propionamido]hexanoate. A flow-type biosensor system was operated optimally at 0.2 M sodium potassium phosphate, pH 7.2 with a minimal matrix effect and the selected flow rate for it was 0.155 ml/min. A biosensor response was detected by measuring a steady-state resonant frequency shift after the response time around 8 min. The frequency shifts obtained were quite specific according to the antibody types and P. aeruginosa strains. The biosensor responses to varying concentrations of the P. aeruginosa cells ranging from 1.3 × 10 7 to 1.3 × 10 8 CFU/ml were determined as 17-176 Hz and a linear calibration curve (r = 0.942) was obtained by plotting the responses in a double-logarithmic scale. The selectivity of the biosensor between P. aeruginosa and Xanthomonas spp. which both b

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Author

Namsoo Kim; In-Seon Park; Dong-Kyung Kim

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0925-4005)

Published

2004

Language

EN

Field

Engineering (Physical Sciences)