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A common nonsense mutation in the repetitive Kringle IV-2 domain of human apolipoprotein(a) results in a truncated protein and low plasma Lp(a): NONSENSE MUTATION IN K IV-2 OF LPA by Walther Parson; Hans Georg Kraft; Harald Niederstätter; Arnulf W. Lingenhel; Silvano Köchl; Friedrich Fresser; Gerd Utermann is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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LPA, the gene coding for apolipoprotein(a) [apo(a)], is the major determinant of lipoprotein(a) [Lp(a)] plasma levels, which are associated with risk for coronary heart disease (CHD) and stroke. It is not completely understood how variation in LPA relates to Lp(a) concentrations. One type of variation related to Lp(a) levels is the number of Kringle (K) IV-2 (g.61C>T; GenBank L14005.1) repeats in LPA, but sequence variation may also contribute. Human apo(a) contains from two to >40 nearly identical K IV-2 repeats of genomic size 5.5 kb, which makes it difficult to detect mutations. To elucidate the genetic variation of the apo(a) K IV-2 domain, we isolated a single "nonexpressing" apo(a) allele with 26 K IV-2 repeats, followed by PCR, cloning and sequencing of 96 clones, resulting in an average coverage of each K IV-2 repeat of approximately four-fold. The previously described K IV types 2A and 2B (K IV-2A and K IV-2B) were detected in 74% of the clones. In addition, a new type designated 2C (K IV-2C) was present. A nonsense mutation in the first exon of K IV-2 (g.61C>T) predicted to result in a truncated protein (p.R21X) was found in nine clones on a K IV-2A background. The presen

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Author

Walther Parson; Hans Georg Kraft; Harald Niederstätter; Arnulf W. Lingenhel; Silvano Köchl; Friedrich Fresser; Gerd Utermann

Publisher

Hindawi Limited

Published

2004

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)