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Effect of D to E mutation of the RGD motif in rhodostomin on its activity, structure, and dynamics: Importance of the interactions between the D residue and integrin by Chiu‐Yueh Chen; Jia‐Hau Shiu; Yao‐Husn Hsieh; Yu‐Chen Liu; Yen‐Chin Chen; Yi‐Chun Chen; Wen‐Yih Jeng; Ming‐Jer Tang; Szecheng J. Lo; Woei‐Jer Chuang is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Effect of D to E mutation of the RGD motif in rhodostomin on its activity, structure, and dynamics: Importance of the interactions between the D residue and integrin about?

## Abstract Rhodostomin (Rho) is a snake venom protein containing an RGD motif that specifically inhibits the integrin‐binding function. Rho produced in __Pichia pastoris__ inhibits platelet aggregation with a K~I~ of 78 n__M__ as potent as native Rho. In contrast, its D51E mutant inhibits platelet aggregation with a K~I~ of 49 μ__M__. Structural analysis of Rho and its D51E mutant showed that they have the same tertiary fold with three two‐stranded antiparallel β‐sheets. There are no structural backbone differences between the RG[D/E] loop which extends outward from the protein core and the RG[D/E] sequence at its apex in a four‐residue RG[D/E]M type I turn. Two minor differences between Rho and its D51E mutant were only found from their backbone dynamics and 3D structures. The R~2~ value of E51 is 13% higher than that of the D51 residue. A difference in the charge separation of 1.76 Å was found between the sidechains of positive (R49) and negative residues (D51 or E51).The docking of Rho into integrin αvβ3 showed that the backbone amide and carbonyl groups of the D51 residue of Rho were formed hydrogen bonds with the integrin residues R216 and R214, respectively. In contrast, the

Who reads Effect of D to E mutation of the RGD motif in rhodostomin on its activity, structure, and dynamics: Importance of the interactions between the D residue and integrin?

Author

Chiu‐Yueh Chen; Jia‐Hau Shiu; Yao‐Husn Hsieh; Yu‐Chen Liu; Yen‐Chin Chen; Yi‐Chun Chen; Wen‐Yih Jeng; Ming‐Jer Tang; Szecheng J. Lo; Woei‐Jer Chuang

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); Wiley-Liss Inc; Wiley (ISSN 0887-3585)

Published

2009

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)