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Biochemical and functional characterization of the Tn-specific lectin from Salvia sclarea seeds by Andrea Medeiros; Sergio Bianchi; Juan J. Calvete; Henia Balter; Sylvie Bay; Ana Robles; Danièle Cantacuzène; Manfred Nimtz; Pedro M. Alzari; Eduardo Osinaga is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Biochemical and functional characterization of the Tn-specific lectin from Salvia sclarea seeds about?
SSL, the lectin isolated from __Salvia sclarea__ seeds, recognizes the Tn antigen (GalNAcα‐O‐Ser/Thr), a specific marker of many human carcinomas. Two‐dimensional electrophoresis, amino‐acid and amino‐sugar analysis, and MALDI‐TOF MS showed that SSL is an acidic (pI 5.5), 60–61‐kDa dimeric glycoprotein composed of apparently identical subunits linked by a single disulfide bond. The apparent molecular mass of SSL in solution determined by equilibrium sedimentation analytical ultracentrifugation was 59 ± 9 kDa. This value did not change in the pH range 2.5–8.5, indicating that SSL does not associate into higher order structures. Tandem mass spectrometry and methylation analysis of N‐glycans released from SSL by hydrazinolysis indicated that SSL possesses 2–3 glycosylation sites occupied with the typical plant glycans Manα1–6[(Manα1–3)(Xylβ1–2)]Manβ1–4‐GlcNAcβ1–4(Fucα1–3)GlcNAc and [(Manα1–3/6)(Xylβ1–2)]Manβ1–4‐GlcNAcβ1–4(Fucα1–3)GlcNAc. The influence of adjacent Tn structures on the binding of two Tn‐specific lectins (SSL and the isolectin B4 from __Vicia villosa__) and an anti‐Tn monoclonal antibody (mAb 83D4) was evaluated using synthetic Tn glycopeptides. The binding of both lecti
Who reads Biochemical and functional characterization of the Tn-specific lectin from Salvia sclarea seeds?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Andrea Medeiros; Sergio Bianchi; Juan J. Calvete; Henia Balter; Sylvie Bay; Ana Robles; Danièle Cantacuzène; Manfred Nimtz; Pedro M. Alzari; Eduardo Osinaga
- Publisher
- John Wiley and Sons; Springer; Wiley (Blackwell Publishing); Springer-Verlag; Blackwell Publishing Inc.; Springer Verlag; Wiley; Springer Science and Business Media LLC (ISSN 1432-1327)
- Published
- 2000
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)