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CD4^+^FOXP3^+^ T Cells in Rheumatoid Arthritis Bone Marrow Are Partially Impaired by Massalska, Magdalena; Radzikowska, Anna; Kuca-Warnawin, Ewa; Plebanczyk, Magdalena; Prochorec-Sobieszek, Monika; Skalska, Urszula; Kurowska, Weronika; Maldyk, Pawel; Kontny, Ewa; Gober, Hans-JÃ1⁄4rgen; Maslinski, Wlodzimierz is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is CD4^+^FOXP3^+^ T Cells in Rheumatoid Arthritis Bone Marrow Are Partially Impaired about?
There is evolving evidence that dysregulation of immune homeostasis in the bone marrow (BM) adjacent to the inflamed joints is involved in the pathogenesis of. In this study, we are addressing the phenotype and function of regulatory T cells (Tregs) residing in the BM of patients with rheumatoid arthritis (RA) and osteoarthritis (OA). BM and peripheral blood samples were obtained from RA and OA patients undergoing hip replacement surgery. The number and phenotype of Tregs were analyzed by flow cytometry and immunohistochemistry. The function of Tregs was investigated ex vivo, addressing their suppressive activity on effector T cells. [^3^H]-Thymidine incorporation assay and specific enzyme-linked immunosorbent assay were used for quantification of cell proliferation and pro-inflammatory (TNF, IFN-γ) cytokine release, respectively. Significantly lower numbers of CD4^+^FOXP3^+^ T cells were found in the BM of patients with RA compared to control patients with OA. High expression of CD127 (IL-7α receptor) and relatively low expression of CXCR4 (receptor for stromal cell-derived factor CXCL12) are characteristics of the CD4^+^FOXP3^+^ cells residing in the BM of RA patients. The BM-res
Who reads CD4^+^FOXP3^+^ T Cells in Rheumatoid Arthritis Bone Marrow Are Partially Impaired?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Massalska, Magdalena; Radzikowska, Anna; Kuca-Warnawin, Ewa; Plebanczyk, Magdalena; Prochorec-Sobieszek, Monika; Skalska, Urszula; Kurowska, Weronika; Maldyk, Pawel; Kontny, Ewa; Gober, Hans-JÃ1⁄4rgen; Maslinski, Wlodzimierz
- Publisher
- Multidisciplinary Digital Publishing Institute (MDPI) (ISSN 2073-4409)
- Published
- 2020
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)