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Antigenal Retrival by Patricia Bocanegra is a document available to read on EtoBox.
This study investigates the effects of pH and ionic strength on heat-induced antigen retrieval (HIAR) in immunohistochemistry, focusing on the antigenicity recovery of various antibodies in formaldehyde-fixed and paraffin-embedded tissues. Results indicate that most antibodies regain antigenicity at both acidic and basic pH levels, with some showing stronger reactions at basic pH. Additionally, ionic strength impacts immunostaining intensity, suggesting that pH and ionic conditions are critical for effectiv
- Author
- Patricia Bocanegra
- Language
- EN