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A Novel Silicon Patch-clamp Chip Permits High-fidelity Recording of Ion Channel Activity from Functionally Defined Neurons by Christophe Py; Mike W. Denhoff; Marzia Martina; Robert Monette; Tanya Comas; Tarun Ahuja; Dolores Martinez; Simon Wingar; Juan Caballero; Sylvain Laframboise; John Mielke; Alexei Bogdanov; Collin Luk; Naweed Syed; Geoff Mealing is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is A Novel Silicon Patch-clamp Chip Permits High-fidelity Recording of Ion Channel Activity from Functionally Defined Neurons about?
## Abstract We report on a simple and high‐yield manufacturing process for silicon planar patch‐clamp chips, which allow low capacitance and series resistance from individually identified cultured neurons. Apertures are etched in a high‐quality silicon nitride film on a silicon wafer; wells are opened on the backside of the wafer by wet etching and passivated by a thick deposited silicon dioxide film to reduce the capacitance of the chip and to facilitate the formation of a high‐impedance cell to aperture seal. The chip surface is suitable for culture of neurons over a small orifice in the substrate with minimal leak current. Collectively, these features enable high‐fidelity electrophysiological recording of transmembrane currents resulting from ion channel activity in cultured neurons. Using cultured Lymnaea neurons we demonstrate whole‐cell current recordings obtained from a voltage‐clamp stimulation protocol, and in current‐clamp mode we report action potentials stimulated by membrane depolarization steps. Despite the relatively large size of these neurons, good temporal and spatial control of cell membrane voltage was evident. To our knowledge this is the first report of record
Who reads A Novel Silicon Patch-clamp Chip Permits High-fidelity Recording of Ion Channel Activity from Functionally Defined Neurons?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Christophe Py; Mike W. Denhoff; Marzia Martina; Robert Monette; Tanya Comas; Tarun Ahuja; Dolores Martinez; Simon Wingar; Juan Caballero; Sylvain Laframboise; John Mielke; Alexei Bogdanov; Collin Luk; Naweed Syed; Geoff Mealing
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); Wiley - V C H Verlag GmbbH & Co.; Wiley (ISSN 0006-3592)
- Published
- 2010
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)