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**Generation of co‐dominant PCR‐based markers by duplex analysis on high resolution gels** by Marie‐Theres Hauser; Farzaneh Adhami; Maria Dorner; Esther Fuchs; Josef Glössl is a Agricultural and Biological Sciences article available to read on EtoBox.

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## Summary Rapid and efficient procedures for the detection of sequence polymorphisms are essential for chromosomal walking and mutation detection analyses. While DNA chip technology and denaturing high‐performance liquid chromatography (DHPLC) are the methods of choice for large scale facilities, small laboratories are dependent on simple ready‐to‐use techniques. We show that heteroduplex analysis on high resolution gel matrices efficiently detects sequence polymorphism differing as little as a single base pair (e.g. single‐nucleotide polymorphism, SNP) with standard laboratory equipment. Furthermore, the matrices also discerned differences between homoduplexes, a prerequisite for co‐dominant markers. The markers thus generated are referred to as duplex analysis markers. We designed PCR primers for 36 __Arabidopsis thaliana__ loci ranging in length from 230 bp to 1000 bp. Among three ecotypes, more than half ( __n__ = 19) of the loci examined were polymorphic; five of which contained three different alleles. This simple, high resolution technique can be used to rapidly convert sequence tagged sites into co‐dominant PCR‐based molecular markers for fine‐scale mapping studies and chr

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Author

Marie‐Theres Hauser; Farzaneh Adhami; Maria Dorner; Esther Fuchs; Josef Glössl

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0960-7412)

Published

1998

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)