Can I read Chromosome-autonomous feedback down-regulates meiotic DNA break competence upon synaptonemal complex formation on EtoBox?

Chromosome-autonomous feedback down-regulates meiotic DNA break competence upon synaptonemal complex formation by Mu, Xiaojing; Murakami, Hajime; Mohibullah, Neeman; Keeney, Scott is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Chromosome-autonomous feedback down-regulates meiotic DNA break competence upon synaptonemal complex formation about?

The number of DNA double-strand breaks (DSBs) initiating meiotic recombination is elevated in Saccharomyces cerevisiae mutants that are globally defective in forming crossovers and synaptonemal complex (SC), a protein scaffold juxtaposing homologous chromosomes. These mutants thus appear to lack a negative feedback loop that inhibits DSB formation when homologs engage one another. This feedback is predicted to be chromosome autonomous, but this has not been tested. Moreover, what chromosomal process is recognized as “homolog engagement” remains unclear. To address these questions, we evaluated effects of homolog engagement defects restricted to small portions of the genome using karyotypically abnormal yeast strains with a homeologous chromosome V pair, monosomic V, or trisomy XV. We found that homolog engagement-defective chromosomes incurred more DSBs, concomitant with prolonged retention of the DSB-promoting protein Rec114, while the rest of the genome remained unaffected. SC-deficient, crossover-proficient mutants ecm11 and gmc2 experienced increased DSB numbers diagnostic of homolog engagement defects. These findings support the hypothesis that SC formation provokes DSB protei

Who reads Chromosome-autonomous feedback down-regulates meiotic DNA break competence upon synaptonemal complex formation?

Author

Mu, Xiaojing; Murakami, Hajime; Mohibullah, Neeman; Keeney, Scott

Publisher

Cold Spring Harbor Laboratory Press; Cold Spring Harbor Laboratory (ISSN 0890-9369)

Published

2020

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)