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A Microbial Biosensor for p-Nitrophenol Using Arthrobacter Sp. by Yu Lei; Priti Mulchandani; Wilfred Chen; Joseph Wang; Ashok Mulchandani is a Engineering article available to read on EtoBox.

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## Abstract This article reports the construction, optimization of performance variables and analytical characterization of a sensitive and selective microbial amperometric biosensor for measurement of __p__‐nitrophenol (PNP), a U.S. Environmental Agency priority pollutant. The biosensor consisted of PNP‐degrading/oxidizing bacteria __Arthrobacter__ sp. JS443 as biological sensing element and a dissolved oxygen electrode as the transducer. The best sensitivity and response time were obtained using a sensor constructed with 1.2 mg dry wt. of cells and operating in pH 7.5, 50 mM citrate‐phosphate buffer. Using these conditions, the biosensor was able to measure as low as 28 ppb (0.2 μM) of PNP selectively without interference from structurally similar compounds, such as phenol, nitrophenols and chlorophenols. The service life of the microbial biosensor is around 5 days when stored in the operating buffer at 4 °C. The applicability to lake water is demonstrated.

Who reads A Microbial Biosensor for p-Nitrophenol Using Arthrobacter Sp.?

Author

Yu Lei; Priti Mulchandani; Wilfred Chen; Joseph Wang; Ashok Mulchandani

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); Wiley - V C H Verlag GmbbH & Co.; Wiley (ISSN 1040-0397)

Published

2003

Language

EN

Field

Engineering (Physical Sciences)