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Early Diagnosis of Arthritis in Mice with Collagen-induced Arthritis, Using a Fluorogenic Matrix Metalloproteinase 3–Specific Polymeric Probe by Ju Hee Ryu; Aeju Lee; Jun-Uk Chu; Heebeom Koo; Chang-Yong Ko; Han Sung Kim; Soo-Young Yoon; Byung-Soo Kim; Kuiwon Choi; Ick Chan Kwon; Kwangmeyung Kim; Inchan Youn is a scholarly article available to read on EtoBox.

What is Early Diagnosis of Arthritis in Mice with Collagen-induced Arthritis, Using a Fluorogenic Matrix Metalloproteinase 3–Specific Polymeric Probe about?

## Objective. Early treatment based on an early diagnosis of rheumatoid arthritis (RA) could halt progression of the disease, but early diagnosis is often difficult. Matrix metalloproteinase 3 (MMP -3 ) is thought to be particularly important in the pathogenesis of RA. The aim of this study was to investigate whether an MMP-3-specific polymeric probe could be used for early diagnosis and for visualizing the progression of arthritis, using a near-infrared fluorescence (NIRF) imaging system. Methods. The MMP-3-specific polymeric probe was developed by conjugating NIRF dye, MMP substrate peptide, and dark quencher to self-assembled chitosan nanoparticles. One hour after intravenous administration of the probe, fluorescent images of mice with collagen-induced arthritis at different stages of disease development were obtained. The correlation between the fluorescence recovered in in vivo imaging when using an MMP-3-specific polymeric probe and up-regulated MMP-3 activity in the joint tissues was evaluated by Western blotting and immunohistochemical staining. Histologic analysis and micro-computed tomography (micro-CT) were also used to assess arthritis progression. Results. A significan

Author

Ju Hee Ryu; Aeju Lee; Jun-Uk Chu; Heebeom Koo; Chang-Yong Ko; Han Sung Kim; Soo-Young Yoon; Byung-Soo Kim; Kuiwon Choi; Ick Chan Kwon; Kwangmeyung Kim; Inchan Youn

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0004-3591)

Published

2011

Language

EN