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DNA typing for identification of some species of Calliphoridae: An interest in forensic entomology by Yvan Malgorn; Raphaël Coquoz is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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To determine precisely post mortem interval, larvae and puparium species found on a corpse have to be identified. Among more than 200 cases examined at the entomology department of the Institut de Recherche Criminelle de la Gendarmerie Nationale, two-thirds concerned corpses less than one month old. Therefore, insects from first and second screwworms are the most frequently found [1]. Some species commonly found in France, such as different Lucilia and Calliphora vicina Robineau-Desvoidy, are easily identifiable at an adult stage, but are almost impossible to differentiate at immature stages when only fragments of puparium or necrosed first instar larvae are available. For this reason, an easy and objective method of identification was thus searched by genetic analysis of these insects. Sequencing of partial gene of sub unit I of cytochrome oxydase has been used to predict restriction sites. Restriction enzyme cleavage of PCR products with Dde I allowed us to differentiate these species.

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Author

Yvan Malgorn; Raphaël Coquoz

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0379-0738)

Published

1999

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)