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Coxsackievirus B3 infection induces anti-flavoprotein antibodies in mice by G. Cicek; T. Vuorinen; I. Stähle; P. Stepanek; N. Freudenberg; R. Brandsch is a Immunology and Microbiology article available to read on EtoBox.

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## SUMMARY Enteroviruses, the most common cause of acute myocarditis, are also supposed aetiological agents of dilated cardiomyopathy. Autoantibodies (anti-M7; Klein & Berg, Clin Exp Immunol 1990; 58:283–92) directed against flavoproteins with covalently bound flavin (αFp-Ab; Otto et al., Clin Exp Immunol 1998; 111:541–2) are detected in up to 30% of sera of patients with myocarditis and idiopathic dilated cardiomyopathy (IDCM). Mice inoculated with a myocarditic variant of coxsackievirus B3 (CVB3) were employed to study the occurrence of serum αFp-Ab following viral infection. The presence of αFp-Ab was analysed by Western blotting with the flavoprotein antigens 6-hydroxy-d-nicotine oxidase (6HDNO) and sarcosine oxidase (SaO). Of 10 sera from CVB3-infected mice, five showed a strong reaction with both antigens. The sera were reactive also to the mitochondrial covalently flavinylated proteins dimethylglycine dehydrogenase and sarcosine dehydrogenase. Sera of non-infected mice did not react with these antigens. A 6HDNO mutant protein with non-covalently bound FAD no longer reacted on Western blots with sera of CVB3-infected mice. Preincubation with FAD abolished or reduced the react

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Author

G. Cicek; T. Vuorinen; I. Stähle; P. Stepanek; N. Freudenberg; R. Brandsch

Publisher

Oxford University Press (OUP)

Published

2000

Language

EN

Field

Immunology and Microbiology (Life Sciences)