Can I read Shape Changes Induced by N-Terminal Platination of Ubiquitin by Cisplatin on EtoBox?

Shape Changes Induced by N-Terminal Platination of Ubiquitin by Cisplatin by Jonathan P. Williams; Hazel I. A. Phillips; Iain Campuzano; Peter J. Sadler is a Chemistry article available to read on EtoBox.

What is Shape Changes Induced by N-Terminal Platination of Ubiquitin by Cisplatin about?

The three-dimensional conformation of a protein is an important property and plays a key role in its biological activity. We show here that ion mobility-mass spectrometry (IM-MS) can be used to detect conformational changes in the protein ubiquitin in the gas phase induced by reaction with the anticancer drug cisplatin. The primary adduct was ubiquitin-{Pt(NH(3))(2)} under denaturing conditions. Up to three different conformations appear to be generated upon platination depending on the charge state. The collision cross-sections (Omega) for each conformation indicate that the conformations of the platinated protein are contracted in size compared with unmodified ubiquitin with generally smaller Omega values. Ion mobility-tandem MS allowed determination of the platinum binding site without a requirement for prior chromatographic separation. A rapid 30-min digestion of cisplatin-modified ubiquitin with trypsin allowed the platination site to be identified as the N-terminal methionine following low-energy collision-induced dissociation (CID) studies of the modified peptide. The data were generated using a Traveling-Wave based ion mobility-MS approach. Such cisplatin-induced shape chan

Who reads Shape Changes Induced by N-Terminal Platination of Ubiquitin by Cisplatin?

Author

Jonathan P. Williams; Hazel I. A. Phillips; Iain Campuzano; Peter J. Sadler

Publisher

Elsevier Science; Springer-Verlag; Springer New York LLC; American Chemical Society (ACS); Springer Science and Business Media LLC; Elsevier BV (ISSN 1044-0305)

Published

2010

Language

EN

Field

Chemistry (Physical Sciences)