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This document describes the development and validation of a high-performance liquid chromatography (HPLC) method for quantifying the major saponin (PFS) in Polyscias fruticosa plant samples. The method uses a C18 column with an isocratic mobile phase of 2% acetonitrile in water containing 0.1% acetic acid. The calibration curve was linear over the test range with an R2 of 0.9996. The method was successfully applied to quantify PFS in P. fruticosa leaves and roots, finding higher levels in the leaves (1300 μ

Author

nguyenanhlam

Language

EN