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Identification and Quantitation of Auxins in Plants by Liquid Chromatography/electrospray Ionization Ion Trap Mass Spectrometry by Qiaomei Lu; Lan Zhang; Tianwen Chen; Minghua Lu; Tong Ping; Guonan Chen is a Chemistry article available to read on EtoBox.

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## Abstract Auxin is an important phylohormone, which regulates specific physiological responses such as division, elongation and differentiation of cells. A new method using liquid chromatography/electrospray ionization ion trap mass spectrometry (LC/ESI‐ITMS) has been developed for identification and quantitation of four auxins. Under the optimum conditions, four auxins (indole‐3‐acetic acid, indole‐3‐propionic acid, indole‐3‐butyric acid and 1‐naphthylacetic acid) were completely separated and quantitated within 7 min with a minimum detection limit of 8.0 ng mL^−1^ with relative standard deviations lower than 5.0%. This method also has been applied to analysis of auxins in Chinese cabbage where, even with a complicated serious background perturbation due to the natural biological matrix, the mean recoveries ranged from 77.5% to 99.8%. Finally, we discuss the MS‐relevant properties of the identified auxins in detail. Copyright © 2008 John Wiley & Sons, Ltd.

Who reads Identification and Quantitation of Auxins in Plants by Liquid Chromatography/electrospray Ionization Ion Trap Mass Spectrometry?

Author

Qiaomei Lu; Lan Zhang; Tianwen Chen; Minghua Lu; Tong Ping; Guonan Chen

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0951-4198)

Published

2008

Language

EN

Field

Chemistry (Physical Sciences)