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Oxidized Low-density Lipoprotein Acts Synergistically with β-Glycerophosphate to Induce Osteoblast Differentiation in Primary Cultures of Vascular Smooth Muscle Cells by Mackenzie Bear; Martin Butcher; Stephen G. Shaughnessy is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract Previous studies have localized osteoblast specific markers to sites of calcified atherosclerotic lesions. We therefore decided to use an established in vitro model of vascular calcification in order to confirm earlier reports of oxidized low‐density lipoprotein (oxLDL) promoting the osteogenic differentiation of vascular smooth muscle cells. Treatment of primary bovine aortic smooth muscle cells (BASMCs) with β‐glycerophosphate was found to induce a time‐dependent increase in osteoblast differentiation. In contrast, no effect was seen when BASMCs were cultured in the presence of oxLDL alone. However, when the BASMCs were cultured in the presence of both β‐glycerophosphate and oxLDL, β‐glycerophosphate's ability to induce osteoblast differentiation was significantly enhanced. In an attempt to resolve the mechanism by which this effect was occurring, we examined the effect of β‐glycerophosphate and oxLDL on several pathways known to be critical to the differentiation of osteoblasts. Surprisingly, β‐glycerophosphate alone was found to enhance Osterix (Osx) expression by inducing both Smad 1/5/8 activation and Runx2 expression. In contrast, oxLDL did not affect either Smad

Who reads Oxidized Low-density Lipoprotein Acts Synergistically with β-Glycerophosphate to Induce Osteoblast Differentiation in Primary Cultures of Vascular Smooth Muscle Cells?

Author

Mackenzie Bear; Martin Butcher; Stephen G. Shaughnessy

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0730-2312)

Published

2008

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)