Can I read Ferricytochrome C Encapsulated in Silica Hydrogels: Correlation Between Active Site Dynamics and Solvent Structure on EtoBox?
Ferricytochrome C Encapsulated in Silica Hydrogels: Correlation Between Active Site Dynamics and Solvent Structure by Maria Grazia Santangelo; Matteo Levantino; Eugenio Vitrano; Antonio Cupane is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Ferricytochrome C Encapsulated in Silica Hydrogels: Correlation Between Active Site Dynamics and Solvent Structure about?
Ferricytochrome c encapsulated in silica hydrogels has been prepared by the sol-gel technique following, with some modifications, the procedure originally developed by Ellerby et al. (Science 255 1113 (1992)). A suitable preparation of hydrogels enables having both 'wet' and 'dry' samples. Wet samples have a high water content: as the temperature is lowered below approximately 260 K, water freezes and the samples crack. On the contrary, dry samples have a low water content (hydration h approximately equal 0.35): in these conditions water does not freeze even at cryogenic temperatures and the samples remain transparent and non-cracking. The dynamics of ferricytochrome c and its dependence on the surrounding medium have been studied by optical absorption spectroscopy in the temperature range 10-300 K. At each temperature, spectra were collected both in the Soret region and in the near infrared at approximately 1.45 microm (the water overtone band); this enables probing the local dynamics of the protein active site as well as the 'structure' of water molecules present in the sample. The data show that sol-gel encapsulation 'per se' does not alter the protein active site dynamics, but
Who reads Ferricytochrome C Encapsulated in Silica Hydrogels: Correlation Between Active Site Dynamics and Solvent Structure?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Maria Grazia Santangelo; Matteo Levantino; Eugenio Vitrano; Antonio Cupane
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 0301-4622)
- Published
- 2003
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)