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Speckle-type POZ protein suppresses hepatocellular carcinoma cell migration and invasion via ubiquitin-dependent proteolysis of SUMO1/sentrin specific peptidase 7 by Ji, Piyou; Liang, Shaohua; Li, Pengtao; Xie, Chengrong; Li, Jie; Zhang, Kang; Zheng, Xuqing; Feng, Min; Li, Qiu; Jiao, Hui; Chi, Xiaoqin; Zhao, Wenxiu; Zhang, Sheng; Wang, Xiaomin is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Speckle-type POZ protein suppresses hepatocellular carcinoma cell migration and invasion via ubiquitin-dependent proteolysis of SUMO1/sentrin specific peptidase 7 about?

Hepatocellular carcinoma (HCC) is associated with high metastatic potential and high mortality. Accumulating evidence has demonstrated that speckle-type POZ protein (SPOP) is a key adaptor molecule of ubiquitination. However, the molecular mechanism of SPOP-mediated ubiquitination in HCC metastasis remains obscure. In the present study, our results indicated that SPOP expression was significantly downregulated in HCC and was associated with tumor size, differentiation and metastasis. Cox regression model showed that low SPOP expression was a risk factor related to the prognosis of HCC patients. Loss- and gain-of-function assays demonstrated that SPOP inhibited HCC cell migration and invasion in vitro. Mechanisitically, co-immunoprecipitation and ubiquitination assays revealed that SPOP recognized and bound SENP7 and promoted its degradation via ubiquitin-dependent proteolysis. Analysis of immunohistochemistry showed that vimentin expression was correlated negatively with SPOP and positively with SENP7. These results implied that increased degradation of SENP7 by overexpression of SPOP decreased vimentin levels, which in turn attenuated HCC cell metastasis. Moreover, in vivo assays

Who reads Speckle-type POZ protein suppresses hepatocellular carcinoma cell migration and invasion via ubiquitin-dependent proteolysis of SUMO1/sentrin specific peptidase 7?

Author

Ji, Piyou; Liang, Shaohua; Li, Pengtao; Xie, Chengrong; Li, Jie; Zhang, Kang; Zheng, Xuqing; Feng, Min; Li, Qiu; Jiao, Hui; Chi, Xiaoqin; Zhao, Wenxiu; Zhang, Sheng; Wang, Xiaomin

Publisher

Elsevier Science; Elsevier ; Elsevier Inc.; Elsevier BV (ISSN 0006-291X)

Published

2018

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)