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Isolation and Characterization of Two Promoters from Linseed for Genetic Engineering by Ravinder K. Jain; R. Gail Thompson; David C. Taylor; Samuel L. MacKenzie; Alan McHughen; Gordon G. Rowland; Don Tenaschuk; Maxine Coffey is a Agricultural and Biological Sciences article available to read on EtoBox.
What is Isolation and Characterization of Two Promoters from Linseed for Genetic Engineering about?
## ABSTRACT Linseed (__Linum usitatissimum__ L.) is an important oilseed crop worldwide and is cultivated for the high level of linolenic acid (18:3) in its seed oil. Currently, there is a concerted effort to improve linseed by genetic engineering. This will require appropriate transgenes and tissue‐specific or constitutive promoters. We report the isolation and characterization of two linseed promoters from a two‐member gene family encoding the enzyme stearoyl‐acyl carrier protein desaturase (SAD). The __SAD1__ and __SAD2__ gene promoter were each fused transcriptionally with the reporter gene for β‐glucuronidase (__uidA__; GUS) and were transferred to linseed to study their expression pattern. In transgenic linseed, GUS activity mediated by the __SAD2__ promoter appeared to be constitutive and was detected in leaves, apices, stem, roots, flower buds, flowers, and seeds. In contrast, GUS activity mediated by the __SAD1__ promoter appeared to be root‐ and seed‐specific. In developing seeds, both the promoters exhibited a temporal expression pattern concomitant with protein and lipid biosyntheses. The GUS activity could be detected as early as 4 days after pollination (dap) and in m
Who reads Isolation and Characterization of Two Promoters from Linseed for Genetic Engineering?
It is typically read by researchers, students, and practitioners in Agricultural and Biological Sciences.
- Author
- Ravinder K. Jain; R. Gail Thompson; David C. Taylor; Samuel L. MacKenzie; Alan McHughen; Gordon G. Rowland; Don Tenaschuk; Maxine Coffey
- Publisher
- The American Society of Agronomy; Crop Science Society of America; Wiley (ISSN 0011-183X)
- Published
- 1999
- Language
- EN
- Field
- Agricultural and Biological Sciences (Life Sciences)