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A Modern Automated Patch-clamp Approach for High Throughput Electrophysiology Recordings in Native Cardiomyocytes by Fitzwilliam Seibertz; Markus Rapedius; Funsho E. Fakuade; Philipp Tomsits; Aiste Liutkute; Lukas Cyganek; Nadine Becker; Rupamanjari Majumder; Sebastian Clauß; Niels Fertig; Niels Voigt is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract Crucial conventional patch-clamp approaches to investigate cellular electrophysiology suffer from low-throughput and require considerable experimenter expertise. Automated patch-clamp (APC) approaches are more experimenter independent and offer high-throughput, but by design are predominantly limited to assays containing small, homogenous cells. In order to enable high-throughput APC assays on larger cells such as native cardiomyocytes isolated from mammalian hearts, we employed a fixed-well APC plate format. A broad range of detailed electrophysiological parameters including action potential, L-type calcium current and basal inward rectifier current were reliably acquired from isolated swine atrial and ventricular cardiomyocytes using APC. Effective pharmacological modulation also indicated that this technique is applicable for drug screening using native cardiomyocyte material. Furthermore, sequential acquisition of multiple parameters from a single cell was successful in a high throughput format, substantially increasing data richness and quantity per experimental run. When appropriately expanded, these protocols will provide a foundation for effective mechanistic an

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Author

Fitzwilliam Seibertz; Markus Rapedius; Funsho E. Fakuade; Philipp Tomsits; Aiste Liutkute; Lukas Cyganek; Nadine Becker; Rupamanjari Majumder; Sebastian Clauß; Niels Fertig; Niels Voigt

Publisher

Springer Science and Business Media LLC

Published

2022

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)