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Determination of Chondroitin Sulfate from Different Sources of Cartilage by W. Garnjanagoonchorn; L. Wongekalak; A. Engkagul is a Engineering article available to read on EtoBox.

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Cartilage is produced as a by-product from slaughter house and fishery industries in Thailand. The enzymatic extraction of chondroitin sulfate followed the method of Nakano et al. [T. Nakano, H.H. Sunwoo, X. Li, M.A. Price, S.S. Jeong, Study of sulfated glycosaminoglycans from porcine skeletal muscle epimysium including analysis of iduronosyl and glucoronosyl residues in galactosaminoglycan fractions, J. Agric. Food Chem. 44 (1996) 1424-1434] and the sulfate glycosaminoglycans (GAGs) assay [W.R. Farndale, D.J. Buttle, A.J. Barrett, Improved quantitation and discrimination of sulphated glycosaminoglycans by use of dimethylmethylene blue, Biochim. Biophys. Acta 883 (1986) 173-177] was employed to determine the content of chondroitin sulfate in cartilage of shark fin, ray, crocodile and chicken keel. Identification of types of chondroitin sulfate through FTIR spectroscopy KBr pellet technique was carried out. The results indicated that chicken keel, crocodile hyoid and sternum cartilage are the most promising potential sources of chondroitin sulfate. The value ranges from 11.55 to 14.84 g/100 g of dried cartilage, calculated as chondroitin-4-sulfate. Identification of dried chondroiti

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Author

W. Garnjanagoonchorn; L. Wongekalak; A. Engkagul

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0255-2701)

Published

2007

Language

EN

Field

Engineering (Physical Sciences)