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Self-assembled Monolayers of Thiols on Gold Electrodes for Bioelectrochemistry and Biosensors by Shaojun Dong; Jinghong Li is a Engineering article available to read on EtoBox.

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Monolayers of biological compounds including redox proteins and enzymes, and phospholipids have been immobilized on a gokl electrnne surface through self-assembling. These proteins ann enzymes, such as cytuchronm c, cytochton~ c oxidase and horseradL~h peroxidase (HRP). immobilized covalenfly to the self-assembled monolayers (SAMs) of 3-mercaptopropionic acid on a gold electrode. communicate directly electrons with the electrode surface witlmut mediators and keep their physiological activities. TI~ e~ ~fer of HRP with the gold electrode can also be mediated by the alkanethiol SAMs with electroactive group viologens on the gold electrode surface. All these direct electrochemistries of proteins and enzymes might offer an opportunity to build a third generation of biosensors without mediators for analytes, such as H202, glucose and cholesterol. Monensin and valinomycin have been incorporated into the bilayers on the gold electrode consisting of the SAMs of alkanethiol and a lipid monolayer, which have high selectivity for rmmovalem ions, and the resulting Na + or K + sensor has a wide linear range and high stability. These self-assembly systems provide a good mimetic model for studyin

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Author

Shaojun Dong; Jinghong Li

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 1567-5394)

Published

1997

Language

EN

Field

Engineering (Physical Sciences)