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IgA induced activation of human mesangial cells: Independent of FcαR1 (CD 89) by Diven, Steven C.; Caflisch, Carlton R.; Hammond, Dianne K.; Weigel, Paul H.; Oka, Janet A.; Goldblum, Randall M. is a Medicine article available to read on EtoBox.

What is IgA induced activation of human mesangial cells: Independent of FcαR1 (CD 89) about?

## Background: Iga nephropathy (igan) is characterized by deposition of polymers of iga1 in the mesangium, accumulation of mesangial matrix and mesangial cell proliferation. activation of the mesangial cell by iga, via an iga receptor, may be an initiating event in the pathology of igan. ## Methods: We examined the ability of radiolabeled, normal serum iga1 to bind human mesangial cells (hmc). activation of hmc by monomeric (miga1) and heat aggregated iga1 (aiga1) was compared by northern analysis of c-jun expression. the expression of fcalphar1 (cd89) mrna on our cultured mesangial cells was also assessed by northern analysis, reverse transcription-polymerase chain reaction (rt-pcr) and flow cytometry. ## Results: 125i-miga1 and 125i-aiga1 bound to hmc in a dose-dependent, saturable manner with similar affinities. there were 1.2 x 10(6) binding sites per cell, with an affinity constant of 2.3 x 10(6) m(-1). aiga1 induced c-jun expression in a time and dose-dependent manner (2.4-fold above baseline after 60 min exposure to aiga1 200 microg/ml) while miga1 had no effect on c-jun expression. no message for cd 89 was detectable in quiescent or aiga1 stimulated hmc by northern analysis

Who reads IgA induced activation of human mesangial cells: Independent of FcαR1 (CD 89)?

Author

Diven, Steven C.; Caflisch, Carlton R.; Hammond, Dianne K.; Weigel, Paul H.; Oka, Janet A.; Goldblum, Randall M.

Publisher

Nature Publishing Group; Elsevier ; Elsevier Inc.; Elsevier BV; ScienceOpen (ISSN 0085-2538)

Published

1998

Language

EN

Field

Medicine (Health Sciences)