Can I read Using rapid diagnostic tests as source of malaria parasite DNA for molecular analyses in the era of declining malaria prevalence on EtoBox?

Using rapid diagnostic tests as source of malaria parasite DNA for molecular analyses in the era of declining malaria prevalence by Deus S Ishengoma; Sudi Lwitiho; Rashid A Madebe; Nyagonde Nyagonde; Ola Persson; Lasse S Vestergaard; Ib C Bygbjerg; Martha M Lemnge; Michael Alifrangis is a Medicine article available to read on EtoBox.

What is Using rapid diagnostic tests as source of malaria parasite DNA for molecular analyses in the era of declining malaria prevalence about?

## Background Malaria prevalence has recently declined markedly in many parts of Tanzania and other sub-Saharan African countries due to scaling-up of control interventions including more efficient treatment regimens (e.g. artemisinin-based combination therapy) and insecticide-treated bed nets. Although continued molecular surveillance of malaria parasites is important to early identify emerging anti-malarial drug resistance, it is becoming increasingly difficult to obtain parasite samples from ongoing studies, such as routine drug efficacy trials. To explore other sources of parasite DNA, this study was conducted to examine if sufficient DNA could be successfully extracted from malaria rapid diagnostic tests (RDTs), used and collected as part of routine case management services in health facilities, and thus forming the basis for molecular analyses, surveillance and quality control (QC) testing of RDTs. ## Methods One hyper-parasitaemic blood sample (131,260 asexual parasites/μl) was serially diluted in triplicates with whole blood and blotted on RDTs. DNA was extracted from the RDT dilution series, either immediately or after storage for one month at room temperature. The extract

Who reads Using rapid diagnostic tests as source of malaria parasite DNA for molecular analyses in the era of declining malaria prevalence?

Author

Deus S Ishengoma; Sudi Lwitiho; Rashid A Madebe; Nyagonde Nyagonde; Ola Persson; Lasse S Vestergaard; Ib C Bygbjerg; Martha M Lemnge; Michael Alifrangis

Publisher

BioMed Central; Springer (Biomed Central Ltd.); London: BioMed Central, 2002-; Springer Science and Business Media LLC; Research Square (ISSN 1475-2875)

Published

2011

Language

EN

Field

Medicine (Health Sciences)