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Mitochondria in human neutrophils mediate killing of Staphylococcus aureus by Kimberly J. Dunham-Snary; Bas GJ. Surewaard; Jeffrey D. Mewburn; Rachel ET. Bentley; Ashley Y. Martin; Oliver Jones; Ruaa Al-Qazazi; Patricia AD. Lima; Paul Kubes; Stephen L. Archer is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Mitochondria in human neutrophils mediate killing of Staphylococcus aureus about?
## Background Neutrophils play a role in innate immunity and are critical for clearance of __Staphylococcus aureus__. Current understanding of neutrophil bactericidal effects is that NADPH oxidase produces reactive oxygen species (ROS), mediating bacterial killing. Neutrophils also contain numerous mitochondria; since these organelles lack oxidative metabolism, their function is unclear. We hypothesize that mitochondria in human neutrophils contribute to the bactericidal capacity of __S. aureus__. ## Methods and Findings: Using human neutrophils isolated from healthy volunteers (n = 13; 7 females, 6 males), we show that mitochondria are critical in the immune response to __S. aureus__. Using live-cell and fixed confocal, and transmission electron microscopy, we show mitochondrial tagging of bacteria prior to ingestion and surrounding of phagocytosed bacteria immediately upon engulfment. Further, we demonstrate that mitochondria are ejected from intact neutrophils and engage bacteria during __vital NETosis__. Inhibition of the mitochondrial electron transport chain at Complex III, but not Complex I, attenuates __S. aureus__ killing by 50 ± 7%, comparable to the NADPH oxidase inhibit
Who reads Mitochondria in human neutrophils mediate killing of Staphylococcus aureus?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Kimberly J. Dunham-Snary; Bas GJ. Surewaard; Jeffrey D. Mewburn; Rachel ET. Bentley; Ashley Y. Martin; Oliver Jones; Ruaa Al-Qazazi; Patricia AD. Lima; Paul Kubes; Stephen L. Archer
- Publisher
- Elsevier BV
- Published
- 2021
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)