Can I read Comparison of Detergent‐based Decellularization Protocols for the Removal of Antigenic Cellular Components in Porcine Aortic Valve on EtoBox?

Comparison of Detergent‐based Decellularization Protocols for the Removal of Antigenic Cellular Components in Porcine Aortic Valve by Xiaohong Liu; Ning Li; Dejun Gong; Cuiping Xia; Zhiyun Xu is a Medicine article available to read on EtoBox.

What is Comparison of Detergent‐based Decellularization Protocols for the Removal of Antigenic Cellular Components in Porcine Aortic Valve about?

## Abstract ## Background Various detergent‐based protocols are used to remove cells and cellular debris in porcine aortic valve (PAV). However, the removal of antigenic cellular components has not been thoroughly elucidated to date. In this study, we used 4 detergent‐based protocols to decellularize PAVs and aimed to evaluate their effects on removing antigenic cellular components. ## Methods Porcine aortic valves were decellularized using sodium dodecyl sulfate (SDS), SDS in combination with sodium deoxycholate (SDS/SD), Triton X‐100, and Triton X‐100 in combination with SD (Triton X‐100/SD), respectively. Untreated PAVs were used as controls. Immunohistochemical and SDS‐polyacrylamide gel electrophoresis (SDS‐PAGE) analyses were performed to determine the removal of antigenic protein components. Histological, biochemical, and biomechanical analyses were performed to determine the preservation and mechanical properties of the extracellular matrix. PAV tissues were implanted subcutaneously in Sprague Dawley rats to evaluate the host immune response. Implanted PAVs were taken out at the indicated time points for histological and immunohistochemical examinations. ## Results All 4 pr

Who reads Comparison of Detergent‐based Decellularization Protocols for the Removal of Antigenic Cellular Components in Porcine Aortic Valve?

Author

Xiaohong Liu; Ning Li; Dejun Gong; Cuiping Xia; Zhiyun Xu

Publisher

Wiley

Published

2018

Language

EN

Field

Medicine (Health Sciences)