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Culture and Molecular Methods as Complementary Tools for Water Quality Management by Anna Pinar-Méndez; Belén Galofré; Anicet R. Blanch; Cristina García-Aljaro is a Environmental Science article available to read on EtoBox.

What is Culture and Molecular Methods as Complementary Tools for Water Quality Management about?

Bacterial communities in a full-scale drinking water treatment plant (DWTP) were characterized using matrix-assisted laser desorption/ionization time of flight mass-spectrometry (MALDI-TOF MS) to identify HPC isolates and the obtained results were compared to 16S rRNA (V4) metabarcoding data acquired in a previous study. Sixty-three samples were collected at nine stages of the potabilization process: river water and groundwater intake, decantation, sand filtration, ozonization, carbon filtration, reverse osmosis, the mixing chamber and post-chlorination drinking water. In total, 1807 bacterial colonies were isolated, 32 % of which were successfully identified to at least the genus level by MALDI-TOF MS using our previously developed Drinking Water Library. Trends in diversity were similar by both approaches, but differences were observed in the detection of taxa, especially at lower hierarchy levels. High bacterial diversity was observed in river and groundwater, where Proteobacteria predominated. The diversity decreased significantly after the chlorination step, where Bacillus sp. (Firmicutes) and an unknown genus of Obscuribacteraceae (Cyanobacteria) were the most prevalent gener

Who reads Culture and Molecular Methods as Complementary Tools for Water Quality Management?

Author

Anna Pinar-Méndez; Belén Galofré; Anicet R. Blanch; Cristina García-Aljaro

Publisher

Elsevier BV

Published

2022

Language

EN

Field

Environmental Science (Physical Sciences)