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Redirecting Lentiviral Vectors by Insertion of Integrin-tageting Peptides Into Envelope Proteins by Kouki Morizono; Nonia Pariente; Yiming Xie; Irvin S. Y. Chen is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Redirecting Lentiviral Vectors by Insertion of Integrin-tageting Peptides Into Envelope Proteins about?

## Abstract ## Background Targeting gene therapy vectors that can home in on desired cell and tissue types __in vivo__ comprise the ultimate gene delivery system. We have previously developed targeting lentiviral vectors by pseudotyping vectors with modified Sindbis virus envelope proteins. The envelope protein contains the Fc‐binding region of protein A (ZZ domain), so the virus can be conjugated with antibodies. The conjugated antibody mediates specific transduction of the cells and tissues expressing the target antigens, both __in vitro__ and __in vivo__. However, more stable conjugation of targeting molecules would be optimal for use in immunocompetent animals, as well as in humans. ## Methods We inserted integrin‐targeting peptides into two sites of the targeting envelope proteins and determined whether the peptides serve as receptor‐binding regions of the envelope proteins and redirect the pseudotyped viruses. ## Results The integrin‐targeting peptides can mediate binding to cells via the interaction with integrins on target cells and transduction. Peptides with a higher binding affinity increase titers of pseudotyped virus. We found two regions on the envelope protein that c

Who reads Redirecting Lentiviral Vectors by Insertion of Integrin-tageting Peptides Into Envelope Proteins?

Author

Kouki Morizono; Nonia Pariente; Yiming Xie; Irvin S. Y. Chen

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1099-498X)

Published

2009

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)